Plasmids from Euryarchaeota

Crenarchaeota and Euryarchaeota in temperate estuarine sediments.

AIMS Application of molecular techniques to ecological studies has unveiled a wide diversity of micro-organisms in natural communities, previously unknown to microbial ecologists. New lineages of Archaea were retrieved from several non-extreme environments, showing that these micro-organisms are present in a large variety of ecosystems. The aim was therefore to assess the presence and diversity...

Replication slippage of the thermophilic DNA polymerases B and D from the Euryarchaeota Pyrococcus abyssi

Replication slippage or slipped-strand mispairing involves the misalignment of DNA strands during the replication of repeated DNA sequences, and can lead to genetic rearrangements such as microsatellite instability. Here, we show that PolB and PolD replicative DNA polymerases from the archaeal model Pyrococcus abyssi (Pab) slip in vitro during replication of a single-stranded DNA template carry...

Contribution of Marine Group II Euryarchaeota to cyclopentyl tetraethers

Isolation and characterization of plasmids from Streptomyces.

In view of the importance of actinomycetes in the production of antibiotics, it would be useful to find plasmids in these organisms in order to construct molecular breeding systems. Since the first discovery of plasmid involvement in antibiotic production", several plasmids associated with the production of antibiotics have been found in Streptomyces (references are listed in AKAGAWA et al.-')....

Rapid extraction of plasmids from Clostridium perfringens.

Two rapid methods were evaluated for their extraction of plasmids from Clostridium perfringens. The first method involved lysis of 1 to 2 ml of C. perfringens culture by treatment with hyaluronidase, lysozyme, and sarcosyl. DNA, extracted with phenol-chloroform, was treated with RNase, boiled, and electrophoresed in a 1.2% agarose gel. The second method involved lysis of 2 ml of culture by lyso...